| # |
Summary |
| 1. |
No developing embryos seen 48 hours after dsRNA injection
|
| |
Injected worms stop producing embryos 48 hours after dsRNA injection and contain no live/developing embryos at this time.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 2. |
Reduced fecundity of injected worm
|
| |
Injected animals lay less than 100 embryos during their lifetime.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 3. |
Osmotically/ pressure sensitive
|
| |
Embryos rupture when placed on a 2% agar pad and covered with a coverslip.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 4. |
Small embryos
|
| |
Embryos are produced that are less than 50% of the normal size of wild-type embryos.
|
|
| Wild Type |
Phenotype |
| Not available |
 |
|
| 5. |
Catastrophic one-cell arrest
|
| |
Embryos fail to divide and arrest as one-cell embryos.
|
|
| Wild Type |
Phenotype |
| Not available |
 |
|
| 6. |
Large cytoplasmic granules
|
| |
Abnormally large lipid granules are observed in the cytoplasm of P0.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 7. |
Excessive blebbing
|
| |
Excessive shaking and movements are seen in the cell membrane or cytoplasm of one-cell or two-cell embryos.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 8. |
Ectopic cleavage furrows
|
| |
Extra cleavage furrows are seen in one or all cells of a one-cell to four-cell cell embryo.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 9. |
Multiple cavities
|
| |
Multiple vesicles, vacuoles, or cavities are seen during early embryogenesis.
|
|
| Wild Type |
Phenotype |
 |
Not available |
|
| 10. |
Slow cell cycle
|
| |
Embryos take longer to divide during the first and second division.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 11. |
Abnormal one-cell shape
|
| |
One-cell embryos have an altered shape.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 12. |
Pseudocleavage absent
|
| |
No pseudocleavage is observed before or during pronuclear migration.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 13. |
Exaggerated pseudocleavage
|
| |
Embryos have a much more pronounced pseudocleavage than normal.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 14. |
Delayed at pronuclear contact
|
| |
Embryos are either delayed or arrested at pronuclear contact.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 15. |
Polar bodies abnormal
|
| |
Aberrant number (fewer or more than two) or size (matches or exceeds size of early PNs) or internalization of polar bodes
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 16. |
Polar body reabsorbed
|
| |
A polar body is absorbed in either the one-cell or two-cell embryo.
|
|
| Wild Type |
Phenotype |
| Not available |
 |
|
| 17. |
Indistinct maternal pronucleus
|
| |
Maternal pronucleus has a blurry appearance. The nuclear envelope does not have a crisp circular shape.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 18. |
Multiple maternal pronuclei
|
| |
One-cell embryos have two or more maternal pronuclei.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 19. |
Multiple paternal pronuclei or aberrant centrosomes
|
| |
More than one paternal pronucleus is present in the one-cell embryo or there is a defect in centrosome structure.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 20. |
Abnormal size of pronucleus
|
| |
Maternal or paternal pronucleus is either too small or too large.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 21. |
Failure to form pronucleus
|
| |
Either the maternal or paternal pronucleus is absent.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 22. |
Paternal pronucleus migrates
|
| |
Instead of the maternal pronucleus migrating to the posterior end of the one-cell embryo, the paternal pronucleus migrates to meet the maternal pronucleus in the anterior end.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 23. |
Pronuclear breakdown asynchronous
|
| |
Instead of breaking down synchronously, the two pronuclei break down asynchronously in the one-cell embryo.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 24. |
Pronuclei fail to migrate
|
| |
Neither of the pronuclei migrate so they never meet.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 25. |
Pronuclei meet more centrally
|
| |
The maternal and paternal pronuclei meet more centrally instead of meeting in the posterior end of the embryo.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 26. |
P0 spindle positioning defect
|
| |
Altered P0 spindle placement causes either a symmetric first division, a division in which P1 is larger than AB, or a division in which the asymmetry is exaggerated such that AB is much larger than normal.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 27. |
P0 spindle absent
|
| |
No mitotic spindle is seen in P0.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 28. |
P0 spindle does not rotate
|
| |
P0 spindle fails to rotate and extends perpendicular to the long axis of the embryo.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 29. |
Delayed P0 spindle rotation
|
| |
P0 spindle rotates late.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 30. |
Loose mitotic furrow
|
| |
Once formed, the mitotic furrow can "slide" laterally.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 31. |
Cytokinesis defect
|
| |
Cells of the embryo attempt to divide but fail to form two daughter cells.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 32. |
Multiple nuclei in 1, 2, or 4-cell embryos
|
| |
Embryos are seen that contain more than one nucleus per cell in either the one-cell, two-cell, or four-cell embryo.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 33. |
Aberrant nuclear reassembly
|
| |
Nuclear envelope does not reassemble properly.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 34. |
Exaggerated asynchrony
|
| |
Asynchrony of the second division is exaggerated and P1 divides more slowly than normal.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 35. |
Reversed asynchrony
|
| |
P1 divides before AB.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 36. |
Synchronous second division
|
| |
AB and P1 divide synchronously.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 37. |
Aberrant AB or P1 spindle orientation
|
| |
The orientation of the spindle is aberrant in either the AB or P1 cell.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 38. |
Division axis of ABa or ABp aberrant
|
| |
ABa or ABp divide in the wrong orientation.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 39. |
P1 aster abnormal
|
| |
The morphology of the P1 aster is abnormal.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 40. |
AB aster resembles P1 aster
|
| |
The morphology of the AB aster resembles that of the P1 aster.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 41. |
Aberrant cell-cell contacts at the four-cell stage
|
| |
ABa, ABp, EMS, or P1 contacts fewer of its sister cells than in wild-type embryos.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 42. |
ABa, ABp and EMS divide synchronously
|
| |
EMS divides at the same time that ABa and ABp divide.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 43. |
EMS divides after and is smaller than P2
|
| |
EMS divides after and is smaller than P2.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 44. |
P2 and EMS divide synchronously
|
| |
P2 and EMS divide synchronously.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 45. |
Nuclei reform next to cell division remnant
|
| |
After division of either one-cell or two-cell embryos, the nuclei reappear next to the cell division remnant.
|
|
| Wild Type |
Phenotype |
 |
 |
|
| 46. |
EMS fails to extend anteriorly
|
| |
EMS fails to extend anteriorly and "hug" ABa.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
| 47. |
EMS extends too far anteriorly
|
| |
EMS extends too far anteriorly at the four-cell stage.
|
|
| Wild Type |
Phenotype |
| Not available |
Not available |
|
